STRIDE™
DNA Damage Response Functional Assays
STRIDE™
DNA Damage Response Functional Assays
Advantages
Functional Biomarker
Unlike standard IF or IHC-based methods that detect the mere presence of proteins, our assays provide information on the functional engagement of DNA repair proteins in genome maintenance mechanisms.
Pharmacodynamic Information
The assays provide an early pharmacodynamic readout of the direct effect synthetically lethal agents exert on the targeted DNA repair pathway.
Enhanced Sensitivity and Specificity
Due to the unparalleled sensitivity and signal amplification even endogenous protein-occupied DNA breaks can be detected and measured with high specificity.
Tailored to Individual Needs
New assays can be built based on our core STRIDE™ technology platform, allowing collaborative biomarker development based on the protein of your interest.
DNA Damage Response Functional Assays
SSBs
Detection of protein-associated single-strand DNA breaks in:
MMR pathway
sSTRIDE™
- PMS2
BER pathway
sSTRIDE™
- SMUG1
Replication stress
sSTRIDE™
- RPA70
Your pathway
sSTRIDE™
- protein of your interest
DSBs
Detection of protein-associated double-strand DNA breaks in:
HR pathway
dSTRIDE™
- RAD51
dSTRIDE™
- RPA70
Your pathway
dSTRIDE™
- protein of your interest
How STRIDE™ works
Proprietary STRIDE™ technology is a fluorescence-based method, which directly labels DNA damage sites and detects individual DNA lesions. Strong signal amplification allows for robust and reliable quantification of DNA damage, which translates into impactful values for your research and business.
Sensitive detection of double-strand breaks associated with a DNA repair marker
Sensitive detection of single-strand breaks associated with a DNA repair marker
LABELING. Direct enzymatic incorporation of nucleotide analogues 
to DNA ends within double-strand breaks.
RECOGNITION. Attachment of a pair of primary antibodies 
to the labelled DNA end and a repair protein 
.
DETECTION. Secondary antibodies 
binding and STRIDE™ signal 
enhancement on closely localized antibody complexes.
Sensitive detection of single-strand breaks associated with a DNA repair marker
LABELING. Enzymatic modification of a single-strand break with modified nucleotides 
.
RECOGNITION. Attachment of a pair of primary antibodies to the incorporated nucleotides and a repair protein 
.
DETECTION. Secondary antibodies binding and strong STRIDE™ signal enhancement on closely localized antibody complexes.
