STRIDE™

DNA Damage Response Functional Assays

These assays offer sensitive measurements of the engagement of specific DNA repair proteins in the mechanisms of DNA strand break repair. Easily customized, they are a valuable tool for learning about MoA of drug candidates and potentially serving as predictive biomarkers in the future.

STRIDE™

DNA Damage Response Functional Assays

These assays offer sensitive measurements of the engagement of specific DNA repair proteins in the mechanisms of DNA strand break repair. Easily customized, they are a valuable tool for learning about MoA of drug candidates and potentially serving as predictive biomarkers in the future.

Advantages


Functional Biomarker

Unlike standard IF or IHC-based methods that detect the mere presence of proteins, our assays provide information on the functional engagement of DNA repair proteins in genome maintenance mechanisms.


Pharmacodynamic Information

The assays provide an early pharmacodynamic readout of the direct effect synthetically lethal agents exert on the targeted DNA repair pathway.


Enhanced Sensitivity and Specificity

Due to the unparalleled sensitivity and signal amplification even endogenous protein-occupied DNA breaks can be detected and measured with high specificity.


Tailored to Individual Needs

New assays can be built based on our core STRIDE™ technology platform, allowing collaborative biomarker development based on the protein of your interest.


DNA Damage Response Functional Assays

All the STRIDE™ measurements can be applied to quantify DNA damage and repair activity in the nucleus and in mitochondria as well as to quantify cytosolic DNA fragments.

SSBs

Detection of protein-associated single-strand DNA breaks in:

MMR pathway

sSTRIDE™

- PMS2

BER pathway

sSTRIDE™

- SMUG1

Replication stress

sSTRIDE™

- RPA70

Your pathway

sSTRIDE™

- protein of your interest

DSBs

Detection of protein-associated double-strand DNA breaks in:

HR pathway

dSTRIDE™

- RAD51

dSTRIDE™

- RPA70

Your pathway

dSTRIDE™

- protein of your interest


We’ve also developed STRIDE™ assays detecting the occurrence of single- (sSTRIDE) and double-strand (dSTRIDE) DNA breaks, regardless of any repair activity.

How STRIDE™ works

Proprietary STRIDE™ technology is a fluorescence-based method, which directly labels DNA damage sites and detects individual DNA lesions. Strong signal amplification allows for robust and reliable quantification of DNA damage, which translates into impactful values for your research and business.

Sensitive detection of double-strand breaks associated with a DNA repair marker

Sensitive detection of single-strand breaks associated with a DNA repair marker

dna image
Step 01

LABELING. Direct enzymatic incorporation of nucleotide analogues  to DNA ends within double-strand breaks.

dna image
Step 02

RECOGNITION. Attachment of a pair of primary antibodies  to the labelled DNA end and a repair protein  .

dna image
Step 03

DETECTION. Secondary antibodies binding and STRIDE™ signal enhancement on closely localized antibody complexes.

dna image

Sensitive detection of single-strand breaks associated with a DNA repair marker

dna image
Step 01

LABELING. Enzymatic modification of a single-strand break with modified nucleotides  .

dna image
Step 02

RECOGNITION. Attachment of a pair of primary antibodies  to the incorporated nucleotides and a repair protein  .

dna image
Step 03

DETECTION. Secondary antibodies binding and strong STRIDE™ signal enhancement on closely localized antibody complexes.

dna image