STRIDE™

DNA Damage
Detection Assays

Our assays offer direct and sensitive detection of the most deleterious type of DNA damage, DNA breaks. Coupled with accurate and objective quantification, these tools are a perfect fit for monitoring the delicate balance between DNA damage and repair.

STRIDE™

DNA Damage
Detection Assays

Our assays offer direct and sensitive detection of the most deleterious type of DNA damage, DNA breaks. Coupled with accurate and objective quantification, these tools are a perfect fit for monitoring the delicate balance between DNA damage and repair.

Advantages


Biologically proximal

STRIDE™ is designed to detect DNA ends directly at the site of DNA damage, providing a measurement that is independent of the DNA Damage Response, in contrast to classic surrogate protein markers.


Individual DNA break levels

STRIDE™ allows to quantify even a single DNA lesion, which translates into unprecedented sensitivity in measurements of the pharmacodynamic effects of drug candidates.


Selectivity you need

With STRIDE™ both single- or double-strand DNA breaks can be quantified, which contributes to better understanding of the mode of action of therapeutics impacting DNA integrity.


Spatially resolved DNA damage

STRIDE™ assays allow you to detect and quantify DNA ends at the sites of DNA breaks or cytosolic DNA fragments in selected subcellular compartments.


DNA Damage
Detection Assays

All the STRIDE™ measurements can be applied to quantify DNA damage and repair activity in the nucleus and in mitochondria as well as to quantify cytosolic DNA fragments.

SSBs

Detection of single-strand DNA breaks

sSTRIDE™

DSBs

Detection of double-strand DNA breaks

dSTRIDE™


We’ve also developed STRIDE™ assays detecting colocalization of DNA breaks and DNA repair proteins. These unique assays report on the status of a DNA repair pathway of interest.

How STRIDE™ works

Proprietary STRIDE™ technology is a fluorescence-based method, which directly labels DNA damage sites and detects individual DNA lesions. Strong signal amplification allows for robust and reliable quantification of DNA damage, which translates into impactful values for your research and business.

Sensitive detection of single-strand DNA breaks by sSTRIDE™

Sensitive detection of double-strand DNA breaks by dSTRIDE™

dna image
Step 01

LABELING. Enzymatic modification of a single-strand break with modified nucleotides  .

dna image
Step 02

RECOGNITION. Attachment of a pair of primary
antibodies  to the incorporated nucleotides.

dna image
Step 03

DETECTION. Secondary antibodies  binding and strong STRIDE™ signal enhancement on closely localized antibody complexes.

dna image

Sensitive detection of double-strand DNA breaks by dSTRIDE™

dna image
Step 01

LABELING. Direct enzymatic incorporation of nucleotide analogues  to DNA ends within double-strand breaks.

dna image
Step 02

RECOGNITION. Attachment of a pair of primary antibodies  to the labelled DNA ends.

dna image
Step 03

DETECTION. Secondary antibodies binding and strong STRIDE™ signal enhancement on closely localized antibody complexes.

dna image