STRIDE™ in DNA Damage Analysis

Introducing STRIDE™, the new “X-ray” in DNA break detection. Embrace a new era with our fluorescence-based assays that let you uncover the specifics of DNA breaks and their repair in situ, with unique precision, specificity and high sensitivity.

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STRIDE™ in DNA Damage Analysis

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Challenge & Our Solutions

CHALLENGE

DNA integrity is important for storing and transferring genetic information through generations. Dysfunction in DNA repair, even a slight disturbance in genome stability, and an increase in DNA damage levels may contribute to infertility, cancer, premature aging, and neurodegenerative diseases.

SOLUTION

STRIDE™ DNA Damage Detection Assays

Our STRIDE™ technology has addressed this challenge, as it allows for a direct detection and accurate quantification of both single- or double-strand DNA breaks, which ultimately will allow to improve efficiency, cost and speed in your drug development efforts.

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SOLUTION

STRIDE™ DNA Damage Response Functional Assays

intoDNA has pioneered functional assays quantifying the subset of DNA breaks associated with a chosen DNA repair mechanism. Our functional DNA repair assays provide accurate measurement of the level of activity of certain repair pathways and have the potential to be further developed as predictive biomarkers for patient selection.

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Superior DNA Break and Damage Response Detection

Innovation in the analysis of DNA breaks

Our technology distinguishes itself from other methods utilized in DNA damage analysis. STRIDE™ directly labels the sites of DNA breaks and stands as the most proximal marker to date. STRIDE™ is customizable to your specific needs, functioning as a selective marker for double- and single-strand breaks, as well as breaks associated with a particular repair mechanism.

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Beyond Immunofluorescence

STRIDE™ helps address problems encountered with immunofluorescence-based techniques commonly employed in DNA damage analysis. Quantifying immunostained DNA damage-associated proteins presents challenges due to poor staining quality and a low signal-to-noise ratio, whereas distinct STRIDE™ foci can be accurately quantified using our in-house built algorithms. Furthermore, STRIDE™ is specific and works independently of complex DNA damage response (DDR) mechanisms, unlike protein markers such as γH2AX and RAD51.

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STRIDE™ Application Areas

DNA Damage Response (DDR)

STRIDE™, with its sensitivity and precision, provides a direct measure of DNA breaks, independent of repair processes. With STRIDE™ you can reveal how well a patient’s tumor responded to or might respond to treatment targeting specific DDR pathways.

Our assays can give you the direct measurement of single-strand DNA breaks and double-strand DNA breaks, and the activity of involved repair pathways.

EXPLORE OUR CASE STUDIES

Case study

Apr 05, 2024

Studying the MoA of the next generation chemotherapeutic LP-284, a small molecule acylfulvene in a chronic myelogenous leukemia cell line model using STRIDE™

Case study

Feb 16, 2024

Studying the MoA of a DDR inhibitor in Patient-Derived Xenograft (PDX) models using STRIDE

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Case study

Jan 19, 2024

Development and validation of a customized, functional STRIDE-based assay to measure Mismatch Repair (MMR) pathway activity in cells

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Radiotherapeutics

Radiopharmaceuticals are a powerful tool in oncology, targeting cancer cells like missiles, directly damaging DNA and creating various lesions including DNA strand breaks. The extent of DNA damage, together with compromised repair systems, determines the success of the therapy. STRIDE™ assays have proven the capacity to reveal the mechanism of action and assess the efficiency of radiopharmaceutical candidates.

EXPLORE OUR CASE STUDIES

Case study

Mar 04, 2024

Studying the effects of alpha and beta-emitters alone and in combination with a DDR inhibitor in a human cell line model

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Immuno-Oncology

High levels of DNA damage in cancer cells can result in increased visibility for the immune system, making these cells prime targets for immunotherapy.

STRIDE™ provides direct measurement of DNA lesions and fragmentation, making it an essential tool in predicting which patient might benefit most from these immune-based therapies. Tracking changes in DNA damage levels can give insights not only into the treatment’s effectiveness, but also into the potential side effects.

Neurodegenerative Diseases

A growing body of evidence points to the crucial role of DNA damage in neurodegenerative diseases.

DNA damage appears in neurons before symptom onset, therefore, STRIDE™ can potentially serve as an early diagnostic marker. The precise measurement our assays can provide is a key component in understanding specific triggers and risk factors, and the tool you need to evaluate the effectiveness of your therapeutic approach.

Basic Oncology Research

While limited DNA damage can fuel uncontrolled cell growth and tumor progression, on an extensive scale it can hold the key to effective therapeutic approaches.

STRIDE™ is a powerful tool for the precise detection of single-strand and double-strand DNA breaks, giving you the possibility to elevate your research and deepen our understanding of the most fundamental aspect of cancer, DNA integrity. By unlocking this information, we can develop better prevention, diagnosis, and treatment strategies.