STRIDE™ to γH2AX Comparison
Explore the distinct advantages that STRIDE™ offers over the most commonly employed marker of DNA damage, the phosphorylated histone gamma H2AX. While γH2AX is widely utilized, it is acknowledged that it is not flawless and merely serves as a proxy marker for DNA breaks.
STRIDE™ to γH2AX Comparison
Explore the distinct advantages that STRIDE™ offers over the most commonly employed marker of DNA damage, the phosphorylated histone gamma H2AX. While γH2AX is widely utilized, it is acknowledged that it is not flawless and merely serves as a proxy marker for DNA breaks.
Compare Markers
STRIDE™
vs
IF γH2AX staining
STRIDE™ foci form exclusively in the presence of a DNA break, independently of the cell cycle, oxidative stress, and other factors.
specificity to a DNA break
γH2AX phosphorylation is dependent on the cell cycle, oxidative stress, and other factors not associated with a DNA break.
STRIDE™ foci form directly at the site of a DNA break, independently of DNA damage response mechanisms and associated proteins.
dependence on DNA repair mechanisms
γH2AX occurs at a distance from a DNA lesion, and the phosphorylation is dependent on the complex DNA damage response mechanisms.
Low signal-to-noise ratio and the enhancement of the fluorescence signal make STRIDE™ foci straightforward to quantify.
precision of quantification
Quantifying γH2AX is challenging due to clustered signal and the high background arising from the basal level of γH2AX.
STRIDE™ can distinguish between different types of DNA lesions (double- and single-strand breaks), including those repaired through specific pathways.
versatility and applicability
γH2AX is conventionally used as a proxy for double-strand DNA breaks and does not differentiate between specific DNA break types.
specificity to a DNA break
— — — — — — — — — — — — —
STRIDE™
STRIDE™ foci form exclusively in the presence of a DNA break, independently of the cell cycle, oxidative stress, and other factors.
IF γH2AX staining
γH2AX phosphorylation is dependent on the cell cycle, oxidative stress, and other factors not associated with a DNA break.
dependence on DNA repair mechanisms
— — — — — — — — — — — — —
STRIDE™
STRIDE™ foci form directly at the site of a DNA break, independently of DNA damage response mechanisms and associated proteins.
IF γH2AX staining
γH2AX occurs at a distance from a DNA lesion, and the phosphorylation is dependent on the complex DNA damage response mechanisms.
precision of quantification
— — — — — — — — — — — — —
STRIDE™
Low signal-to-noise ratio and the enhancement of the fluorescence signal make STRIDE™ foci straightforward to quantify.
IF γH2AX staining
Quantifying γH2AX is challenging due to clustered signal and the high background arising from the basal level of γH2AX.
versatility and applicability
— — — — — — — — — — — — —
STRIDE™
STRIDE™ can distinguish between different types of DNA lesions (double- and single-strand breaks), including those repaired through specific pathways.
IF γH2AX staining
γH2AX is conventionally used as a proxy for double-strand DNA breaks and does not differentiate between specific DNA break types.
Discover the STRIDE™ Advantage: Side-by-Side Comparison
Images legend
STRIDE™ signal
γH2AX signal
CellMask signal™STRIDE™ foci are straightforward to quantify in contrast to the often seen nuclear-wide γH2AX.
DLD-1 cells treated with hydroxyurea show high level of dSTRIDE™ foci. High signal-to-noise ratio of STRIDE™ technology allows precise and unbiased quantification of DSBs. On the other hand, nuclear-wide pattern of γH2AX without distinct foci makes the quantification challenging.
STRIDE™ foci occur only directly at the sites of DNA damage. H2AX phosphorylation can occur independently from DSB formation, for example in response to oxidative stress.
DLD-1 cells treated with H2O2 show a high level of γH2AX that is not associated with DSB formation as indicated by no correlation with dSTRIDE™ nor 53BP1 foci (data not shown).
STRIDE™ gives information on DNA breaks in the nucleus and extra insight into DNA fragments in other cell compartments.
DLD-1 cells treated with olaparib show an increased level of dSTRIDE™ foci in the cytoplasm , giving valuable information about DNA fragmentation that cannot be concluded from the γH2AX staining.
