STRIDE™ to γH2AX Comparison

Explore the table below to uncover the distinct advantages that STRIDE™ offers over the most commonly employed marker of DNA damage, the phosphorylated histone gamma H2AX. While γH2AX is widely utilized, it is acknowledged that it is not flawless and merely serves as a proxy marker for DNA breaks.
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STRIDE™ to γH2AX Comparison

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Explore the table below to uncover the distinct advantages that STRIDE™ offers over the most commonly employed marker of DNA damage, the phosphorylated histone gamma H2AX. While γH2AX is widely utilized, it is acknowledged that it is not flawless and merely serves as a proxy marker for DNA breaks.

Compare Markers

STRIDE™

vs

IF γH2AX staining

STRIDE™ foci form exclusively in the presence of a DNA break, independently of the cell cycle, oxidative stress, and other factors.
specificity to a DNA break

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γH2AX phosphorylation is dependent on the cell cycle, oxidative stress, and other factors not associated with a DNA break.
STRIDE™ foci form directly at the site of a DNA break, independently of DNA damage response mechanisms and associated proteins.
dependence on DNA repair mechanisms

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γH2AX occurs at a distance from a DNA lesion, and the phosphorylation is dependent on the complex DNA damage response mechanisms.
High signal-to-noise ratio and the enhancement of the fluorescence signal make STRIDE™ foci straightforward to quantify.
precision of quantification

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γH2AX is conventionally used as a proxy for double-strand DNA breaks and do not differentiate between specific DNA break types.
STRIDE™ can distinguish between different types of DNA lesions (double- and single-strand breaks), including those repaired through specific pathways.
versatility and applicability

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γH2AX is conventionally used as a proxy for double-strand DNA breaks and do not differentiate between specific DNA break types.

specificity to a DNA break

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— — — — — — — — — — — — —

STRIDE™

STRIDE™ foci form exclusively in the presence of a DNA break, independently of the cell cycle, oxidative stress, and other factors.

IF γH2AX staining

γH2AX phosphorylation is dependent on the cell cycle, oxidative stress, and other factors not associated with a DNA break.

dependence on DNA repair mechanisms

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— — — — — — — — — — — — —

STRIDE™

STRIDE™ foci form directly at the site of a DNA break, independently of DNA damage response mechanisms and associated proteins.

IF γH2AX staining

γH2AX occurs at a distance from a DNA lesion, and the phosphorylation is dependent on the complex DNA damage response mechanisms.

precision of quantification

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— — — — — — — — — — — — —

STRIDE™

High signal-to-noise ratio and the enhancement of the fluorescence signal make STRIDE™ foci straightforward to quantify.

IF γH2AX staining

γH2AX is conventionally used as a proxy for double-strand DNA breaks and do not differentiate between specific DNA break types.

versatility and applicability

element icon
— — — — — — — — — — — — —

STRIDE™

STRIDE™ can distinguish between different types of DNA lesions (double- and single-strand breaks), including those repaired through specific pathways.

IF γH2AX staining

γH2AX is conventionally used as a proxy for double-strand DNA breaks and do not differentiate between specific DNA break types.

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