A Stride in DNA Break Analysis

Our pioneering technology provides invaluable insight into individual single- and double-strand DNA lesions.

A Stride in DNA Break Analysis

Our pioneering technology provides invaluable insight into individual single- and double-strand DNA lesions.
CHALLENGE AND OPPORTUNITY
DNA integrity is important for storing and transferring genetic information through generations. Dysfunction in DNA repair, even a slight disturbance in genome stability, and an increase in DNA damage levels may contribute to infertility, cancer, premature aging and neurodegenerative diseases.
Reliable and precise measurement of DNA break levels is imperative to the development of effective therapies, yet impossible to achieve with current tools. We aim to target this unaddressed challenge with our technology and accelerate drug development efforts.

STRIDE

SensiTive Recognition
of Individual DNA Ends

 

STRIDE is the first platform technology for the direct detection of DNA ends. It outcompetes all other tools in its sensitivity, providing a reliable measure of DNA damage at an individual lesion level. STRIDE selectively marks either single- or double-strand DNA breaks. It tracks even subtle elevations in DNA break levels, which translates into a better understanding of drug pharmacodynamic effects, and their molecular mechanisms of action.

 

The exceptional qualities of our technology are derived from direct fluorescent labelling of free DNA ends, strong signal amplification, and eliminating any nonspecific background.

 

STRIDE is a versatile method that enables selective DNA break detection in cell nuclei or DNA fragments in the cytoplasm. The labelling can be combined with immunofluorescent staining of various DNA repair process markers, signalling proteins and other subcellular structures. The STRIDE assay itself can be personalised to provide a reliable tool for the analysis of any DNA repair pathway of interest.

 

Currently At

Currently, our clients rely on STRIDE to address research and clinical challenges in three main domains:

DNA Damage Response

STRIDE, with its sensitivity and precision, provides direct measure of DNA breaks, independently of repair processes

Nuclear Medicine

STRIDE selectively marks single- and double-strand DNA breaks to provide insight on mechanisms of action

Immunotherapy

STRIDE detects cytosolic DNA fragments to measure drugs’ acute effect and the immune response of cells

Where DNA Breaks
Our Insight Begins

Applications

Labelling with STRIDE can be performed on a variety of biological material such as fixed cultured cells, frozen and formalin-fixed paraffin-embedded tissue sections, isolated blood cells, tissue microarrays, germ cells, blood smears and can be adapted to new biological material.

Our Process

We approach DNA break detection end-to-end, i.e. from laboratory cell work and tissue preparation, performing the assay and microscopy imaging, to data analysis with our automated machine learning based approach, and comprehensive result interpretation. With our input, our partners get broader insight into their drugs’ mechanism of action, efficiency, pharmacodynamics and safety.

Scalability

STRIDE is a game-changer in any domain where DNA integrity matters. It can be applied in many fields to assess off-target effects or screen genetic material quality (such as in gene editing, reproductive medicine and agriculture among many others). From a technological point of view, since STRIDE is based on fluorescence, it can be scaled to various detection strategies, such as fluorescence and confocal microscopy, flow cytometry or high-throughput screening. It also has potential to be established as a predictive biomarker and a companion diagnostic in targeted cancer therapies.

Our Technology Portfolio

sSTRIDE

for single-strand DNA break detection

dSTRIDE

for double-strand DNA break detection

dSTRIDE-HR

for Rad51-associated double-strand DNA break detection

dSTRIDE-RPA

for RPA-associated double-strand DNA break detection

dSTRIDE

for detection of cytosolic DNA fragments

Our Technology Portfolio

sSTRIDE

for single-strand DNA break detection

dSTRIDE

for double-strand DNA break detection

dSTRIDE-HR

for Rad51-associated double-strand DNA break detection

dSTRIDE-RPA

for RPA-associated double-strand DNA break detection

dSTRIDE

for detection of cytosolic DNA fragments
GLOBAL PERSPECTIVE

Our Partners Around the Globe

Expertise Behind STRIDE

Our technology, expertise, and in-house automation in DNA damage detection translates into value for our clients by accelerating time-to-market for therapeutics.